3S5J
2.0A Crystal structure of human phosphoribosyl pyrophosphate synthetase 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-09-22 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | H 3 |
Unit cell lengths | 170.536, 170.536, 61.778 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.230 - 2.020 |
R-factor | 0.21683 |
Rwork | 0.215 |
R-free | 0.25607 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hcr |
RMSD bond length | 0.008 |
RMSD bond angle | 1.149 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 85.270 |
High resolution limit [Å] | 2.020 |
Number of reflections | 41673 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | VAPOR DIFFUSION, HANGING DROP |