3S3W

Structure of chicken acid-sensing ion channel 1 at 2.6 a resolution and ph 7.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-08-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	110.469, 142.689, 159.736
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.937 - 2.600
R-factor	0.2132
Rwork	0.212
R-free	0.23640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ij4
RMSD bond length	0.003
RMSD bond angle	0.789
Data reduction software	XDS ((VERSION May 10)
Data scaling software	SADABS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.000	2.700
High resolution limit [Å]	2.600	2.600
Rmerge	0.157	0.825
Number of reflections	78139
<I/σ(I)>	8.59	1.18
Completeness [%]	99.8	99.4
Redundancy	6.72	6.61

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	286	0.1M Tris-HCL pH7.5, 0.1M ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 286K