3S3E
Crystal structure of the catalytic domain of PTP10D from Drosophila melanogaster
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-21 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.95 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 102.460, 102.460, 171.842 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.000 - 2.400 |
| R-factor | 0.20566 |
| Rwork | 0.205 |
| R-free | 0.22724 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ahs |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.178 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.000 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.119 | 0.484 |
| Number of reflections | 41551 | |
| <I/σ(I)> | 15.9 | 5.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.2 | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 15% PEG 4000, 120mM Citrate, 10% iso-propanol, 10% n-Butanol, 10% 1,4-Butanediol , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
