3S1V
Transaldolase from Thermoplasma acidophilum in complex with D-fructose 6-phosphate Schiff-base intermediate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.91841 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 148.617, 172.535, 100.277 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.745 - 1.800 |
| R-factor | 0.1768 |
| Rwork | 0.175 |
| R-free | 0.21150 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3s0c |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.275 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.750 | 1.900 | |
| High resolution limit [Å] | 1.800 | 30.000 | 1.800 |
| Rmerge | 0.067 | 0.543 | |
| Number of reflections | 118736 | 17640 | |
| <I/σ(I)> | 20.34 | 4.39 | |
| Completeness [%] | 99.8 | 99.9 | |
| Redundancy | 8.23 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 4.5 | 291 | PEG 6000, GOL, pH 4.5, hanging drop, temperature 291K |
