3S0X
The crystal structure of GxGD membrane protease FlaK
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.895, 99.715, 118.436 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.560 - 3.600 |
| R-factor | 0.27602 |
| Rwork | 0.270 |
| R-free | 0.32713 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.611 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.730 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Number of reflections | 10106 | |
| Completeness [%] | 99.0 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 9.5 | 298 | 30% PEG300 50 mM glycine 100 mM NaCl, pH 9.5, EVAPORATION, temperature 298K |
