3S0K
Crystal Structure of Human Glycolipid Transfer Protein complexed with glucosylceramide containing oleoyl acyl chain (18:1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 273 |
Detector technology | CCD |
Collection date | 2008-09-17 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.659, 61.646, 67.214 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.400 |
R-factor | 0.152 |
Rwork | 0.150 |
R-free | 0.18200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.017 |
RMSD bond angle | 1.721 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.450 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.052 | |
Number of reflections | 41140 | |
Completeness [%] | 98.2 | 90.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 273 | 20-25% PEG 3350 , 0.1M MES pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 273K |