Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 83 |
| Detector technology | CCD |
| Collection date | 2010-02-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.963937, 0.979163, 0.979328 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 164.656, 164.656, 273.458 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.965 - 4.000 |
| R-factor | 0.2732 |
| Rwork | 0.270 |
| R-free | 0.33990 |
| Structure solution method | MAD |
| RMSD bond length | 0.048 |
| RMSD bond angle | 1.747 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 4.302 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.056 | 0.056 |
| Number of reflections | 13319 | |
| <I/σ(I)> | 25.3 | 25.3 |
| Completeness [%] | 97.3 | 97.3 |
| Redundancy | 6.9 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.6 | 277 | 1.8M K/Na phosphate, pH 5.6, VAPOR DIFFUSION, temperature 277K |
