3RYT
The Plexin A1 intracellular region in complex with Rac1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-07 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 110.783, 110.783, 265.628 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.544 - 3.582 |
R-factor | 0.2703 |
Rwork | 0.270 |
R-free | 0.33970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 3IG3 AND 1MH1 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.494 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.568 | 3.730 |
High resolution limit [Å] | 3.582 | 3.600 |
Number of reflections | 20090 | |
Completeness [%] | 99.6 | 98.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 15% PEG1500, 100 mM SPG buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |