3RY9
Crystal Structure of the Resurrected Ancestral Glucocorticoid Receptor 1 in complex with DOC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-08 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 139.421, 49.144, 100.369 |
Unit cell angles | 90.00, 105.82, 90.00 |
Refinement procedure
Resolution | 96.670 - 1.950 |
Rwork | 0.181 |
R-free | 0.21607 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2q3y |
RMSD bond length | 0.009 |
RMSD bond angle | 1.093 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 96.674 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Number of reflections | 48012 | |
<I/σ(I)> | 13 | |
Completeness [%] | 93.9 | 78.3 |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 273 | 2.5-2.8 M sodium acetate trihydrate, 0.1 M Bis-Tris propane, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 273K |