3RW9
Crystal Structure of human Spermidine Synthase in Complex with decarboxylated S-adenosylhomocysteine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-09 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.177, 60.816, 87.021 |
Unit cell angles | 90.00, 108.05, 90.00 |
Refinement procedure
Resolution | 40.560 - 2.000 |
R-factor | 0.2134 |
Rwork | 0.211 |
R-free | 0.25930 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | REFMAC |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.000 |
Number of reflections | 33549 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 295 | 20% PEG 3350, 0.2 M sodium sulfate, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K |