3RVD
Crystal structure of the binary complex, obtained by soaking, of photosyntetic a4 glyceraldehyde 3-phosphate dehydrogenase (gapdh) with cp12-2, both from arabidopsis thaliana.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-09-09 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.934 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 153.183, 188.750, 312.170 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 94.610 - 2.700 |
R-factor | 0.247 |
Rwork | 0.247 |
R-free | 0.31800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3k2b |
RMSD bond length | 0.009 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 95.300 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.098 | 0.587 |
Number of reflections | 118509 | |
<I/σ(I)> | 16.3 | 2.1 |
Completeness [%] | 96.6 | 84 |
Redundancy | 11.3 | 7.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2.4 M ammonium sulphate, 0.1 M sodium citrate, 1 mM NAD, 3.125 mg/ml CP12 for soaking experiments, VAPOR DIFFUSION, SITTING DROP, temperature 293K |