3RTR
A RING E3-substrate complex poised for ubiquitin-like protein transfer: structural insights into cullin-RING ligases
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2010-06-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 88.475, 119.796, 231.861 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.949 - 3.210 |
Rwork | 0.238 |
R-free | 0.28250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ldj |
RMSD bond length | 0.007 |
RMSD bond angle | 0.992 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 3.210 |
Number of reflections | 41039 |
<I/σ(I)> | 21.7 |
Completeness [%] | 99.9 |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 17% PEG 3350, 100 mM Tris pH 7.5, 200 mM Sodium/Postassium Tartrate, 2% Benzamidine Hydrochloride, 1% Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |