3RSW

Crystal Structure of Heart Fatty Acid Binding Protein (FABP3)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	80
Detector technology	CCD
Collection date	2011-04-28
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9774
Spacegroup name	P 43
Unit cell lengths	42.472, 42.472, 168.866
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.480 - 2.599
R-factor	0.22829
Rwork	0.226
R-free	0.27306
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.862
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall
Low resolution limit [Å]	84.400
High resolution limit [Å]	2.599
Rmerge	0.167
Number of reflections	9216
<I/σ(I)>	11.8
Completeness [%]	99.9
Redundancy	11.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	3.2	298	0.1M Citric Acid, pH 3.2 and 32% PEG400, VAPOR DIFFUSION, SITTING DROP, temperature 298K