3RPI
Crystal Structure of Fab from 3BNC60, Highly Potent anti-HIV Antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-02-15 |
| Detector | PSI PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.620, 155.680, 74.760 |
| Unit cell angles | 90.00, 110.40, 90.00 |
Refinement procedure
| Resolution | 39.170 - 2.648 |
| R-factor | 0.2102 |
| Rwork | 0.208 |
| R-free | 0.25760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.309 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.170 | 2.720 |
| High resolution limit [Å] | 2.648 | 2.650 |
| Number of reflections | 38111 | |
| <I/σ(I)> | 15.7 | 2.5 |
| Completeness [%] | 96.0 | 68.1 |
| Redundancy | 5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 11.7% PEG20000, 0.1 M sodium acetate, pH 5.0, 100 mM potassium/sodium tartrate, 20 mM lithium sulfate, 10 mM CHES, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
