3RPI
Crystal Structure of Fab from 3BNC60, Highly Potent anti-HIV Antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-02-15 |
Detector | PSI PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 63.620, 155.680, 74.760 |
Unit cell angles | 90.00, 110.40, 90.00 |
Refinement procedure
Resolution | 39.170 - 2.648 |
R-factor | 0.2102 |
Rwork | 0.208 |
R-free | 0.25760 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.309 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.170 | 2.720 |
High resolution limit [Å] | 2.648 | 2.650 |
Number of reflections | 38111 | |
<I/σ(I)> | 15.7 | 2.5 |
Completeness [%] | 96.0 | 68.1 |
Redundancy | 5 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 11.7% PEG20000, 0.1 M sodium acetate, pH 5.0, 100 mM potassium/sodium tartrate, 20 mM lithium sulfate, 10 mM CHES, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |