3RP7
Crystal Structure of Klebsiella pneumoniae HpxO complexed with FAD and uric acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-08-18 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9791 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.023, 67.840, 82.236 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.920 - 2.042 |
R-factor | 0.194 |
Rwork | 0.192 |
R-free | 0.23180 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.005 |
RMSD bond angle | 1.035 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | REFMAC |
Refinement software | PHENIX (1.7_650) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.110 |
High resolution limit [Å] | 2.040 | 4.390 | 2.040 |
Rmerge | 0.093 | 0.068 | 0.311 |
Number of reflections | 24401 | ||
<I/σ(I)> | 22.8 | ||
Completeness [%] | 99.7 | 98.9 | 99.3 |
Redundancy | 5.5 | 5 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 291 | 0.05 M KH2PO4, 20% PEG 8000, hanging drop, temperature 291K |