3RMX
Crystal structure of HCR/D F1240A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-18 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 94.570, 115.630, 107.240 |
Unit cell angles | 90.00, 91.91, 90.00 |
Refinement procedure
Resolution | 36.610 - 2.750 |
R-factor | 0.239 |
Rwork | 0.239 |
R-free | 0.29300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3n7j |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MERLOT |
Refinement software | CNS (1.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.610 | 2.800 |
High resolution limit [Å] | 2.750 | 2.750 |
Number of reflections | 59660 | |
<I/σ(I)> | 14.7 | 2.7 |
Completeness [%] | 99.0 | 90.2 |
Redundancy | 4 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 292 | 0.1 M MOPS pH 7.0, 10% PEG4K, 12.5% MPD, 100 mM NaCl, VAPOR DIFFUSION, temperature 292K |