3RMD
Crystal Structure of a replicative DNA polymerase bound to DNA containing Thymine Glycol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.03320 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 132.837, 123.025, 168.775 |
| Unit cell angles | 90.00, 95.87, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.980 |
| Rwork | 0.220 |
| R-free | 0.27540 |
| Starting model (for MR) | 2dy4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.277 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.090 |
| High resolution limit [Å] | 2.980 | 6.420 | 2.980 |
| Rmerge | 0.092 | 0.046 | 0.409 |
| Number of reflections | 109082 | ||
| <I/σ(I)> | 15.9 | ||
| Completeness [%] | 98.1 | 99.8 | 82.5 |
| Redundancy | 4.6 | 4.7 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 297 | 4% PEG 20,000, 100 mM Na acetate, 125 mM Mg acetate, 100 mM TrisHCl, 1% glycerol, 10mM Phenol, 2 mM beta-mercaptoethanol, vapor diffusion, hanging drop, temperature 297K, pH 6.8, VAPOR DIFFUSION, HANGING DROP |
