3RI0
Epitope backbone grafting by computational design for improved presentation of linear epitopes on scaffold proteins
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 93 |
Detector technology | IMAGE PLATE |
Collection date | 2010-04-08 |
Detector | MAR scanner 345 mm plate |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 61.600, 80.400, 67.300 |
Unit cell angles | 90.00, 111.90, 90.00 |
Refinement procedure
Resolution | 17.000 - 2.250 |
Rwork | 0.217 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2cx5 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 17.000 | 2.300 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 14246 | |
<I/σ(I)> | 12.8 | 3.5 |
Completeness [%] | 98.0 | 99.5 |
Redundancy | 1.57 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.2 M ammonium sulfate, 30% PEG8000, 0.2 M sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |