3RHU
Epitope backbone grafting by computational design for improved presentation of linear epitopes on scaffold proteins
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-02-01 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | I 4 |
| Unit cell lengths | 103.650, 103.650, 85.669 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.300 - 2.800 |
| R-factor | 0.28182 |
| Rwork | 0.280 |
| R-free | 0.31223 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wnu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.109 |
| Data reduction software | HKL-2000 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.300 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 11260 | |
| <I/σ(I)> | 26.7 | 5.2 |
| Completeness [%] | 99.8 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 2.8 M sodium chloride, 0.1 M Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
