3RHP
Crystal structure of the C707A mutant of the C-Terminal domain of 10'FORMYLTETRAHYDROFOLATE DEHYDROGENASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-08-27 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 261.400, 194.900, 97.110 |
| Unit cell angles | 90.00, 108.70, 90.00 |
Refinement procedure
| Resolution | 48.970 - 2.500 |
| R-factor | 0.21489 |
| Rwork | 0.213 |
| R-free | 0.25697 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o2p |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.089 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.290 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.152 | 0.438 |
| Number of reflections | 155638 | |
| <I/σ(I)> | 7.4 | 2.9 |
| Completeness [%] | 98.2 | 96.7 |
| Redundancy | 4.8 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 293 | 0.1M MES, pH 6.6, 1.8 M Ammonium Sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
