3RGV

A single TCR bound to MHCI and MHC II reveals switchable TCR conformers

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-01-01
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	I 2 2 2
Unit cell lengths	118.872, 146.409, 168.565
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.430 - 2.900
R-factor	0.2046
Rwork	0.202
R-free	0.25440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3C60 and 2ZSV
RMSD bond length	0.010
RMSD bond angle	1.348
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	19.430
High resolution limit [Å]	2.900
Number of reflections	30372

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop	7	298	PEG 3350, pH 7, hanging drop, temperature 298K