3RGD
Iron loaded frog M ferritin. Short soaking time
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-12-10 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.740 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 210.744, 210.744, 322.036 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.537 - 2.890 |
| R-factor | 0.1751 |
| Rwork | 0.172 |
| R-free | 0.22860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rbc |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.097 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 3.050 |
| High resolution limit [Å] | 2.890 | 2.890 |
| Rmerge | 0.182 | |
| Number of reflections | 184115 | |
| <I/σ(I)> | 3.9 | 3.8 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.6 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 7 mg/mL frog M ferritin solution at pH 7.5 with TrisHCl. Precipitant: 2.1M sodium formate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
