3RFU
Crystal structure of a copper-transporting PIB-type ATPase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-06-20 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 |
Unit cell lengths | 44.150, 72.980, 329.950 |
Unit cell angles | 89.96, 90.04, 90.22 |
Refinement procedure
Resolution | 19.996 - 3.200 |
R-factor | 0.236 |
Rwork | 0.235 |
R-free | 0.26060 |
Structure solution method | MIRAS |
RMSD bond length | 0.008 |
RMSD bond angle | 1.096 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHARP |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.000 | 3.300 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 79677 | |
Completeness [%] | 96.0 | 93 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 6 % (w/v) PEG6K, 10 % (v/v) Glycerol, 140 mM NaCl, 3 % v/v t-BuOH, 5 mM BME, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 6 % (w/v) PEG6K, 10 % (v/v) Glycerol, 140 mM NaCl, 3 % v/v t-BuOH, 5 mM BME, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |