3REV
Crystal structure of human alloreactive tcr nb20
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-16 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.240, 72.770, 96.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.000 - 2.200 |
| R-factor | 0.232 |
| Rwork | 0.232 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.227 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.068 | 0.539 |
| Number of reflections | 24266 | |
| <I/σ(I)> | 7.949 | 1.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 298 | 22% PEG 4000, 0.2M AMMONIUM ACETATE, 0.1M CITRIC ACID, PH 4, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 298K |
