3REO
Monolignol O-methyltransferase (MOMT)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-10 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.241, 150.754, 68.302 |
| Unit cell angles | 90.00, 92.67, 90.00 |
Refinement procedure
| Resolution | 44.370 - 1.900 |
| R-factor | 0.19 |
| Rwork | 0.188 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kyz |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.106 |
| Data reduction software | PROCESS |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.110 | 0.380 |
| Number of reflections | 103127 | |
| Completeness [%] | 97.1 | 96.9 |
| Redundancy | 2.6 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | 295 | 26% PEG 4000, 0.3M magnesium nitrate,3% ethylene glycol, and 2 mM DTT , pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
