3REK
2.6 Angstrom Crystal Structure of the Nucleosome Core Particle Assembled with a 146 bp Alpha-Satellite DNA (NCP146b) Derivatized with Oxaliplatin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.07 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 105.001, 109.436, 176.357 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.000 - 2.600 |
| R-factor | 0.26046 |
| Rwork | 0.260 |
| R-free | 0.28120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kx4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.433 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 57.000 |
| High resolution limit [Å] | 2.600 |
| Rmerge | 0.061 |
| Number of reflections | 57610 |
| <I/σ(I)> | 18.4 |
| Completeness [%] | 93.5 |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
