3REH
2.5 Angstrom Crystal Structure of the Nucleosome Core Particle Assembled with a 145 bp Alpha-Satellite DNA (NCP145)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.07 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 105.606, 110.066, 181.719 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 91.000 - 2.500 |
R-factor | 0.23701 |
Rwork | 0.236 |
R-free | 0.26972 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2nzd |
RMSD bond length | 0.009 |
RMSD bond angle | 1.420 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 91.000 |
High resolution limit [Å] | 2.500 |
Rmerge | 0.059 |
Number of reflections | 72054 |
<I/σ(I)> | 21.6 |
Completeness [%] | 99.6 |
Redundancy | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | KCl, MnCl2, K-Cacodylate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |