3REH

2.5 Angstrom Crystal Structure of the Nucleosome Core Particle Assembled with a 145 bp Alpha-Satellite DNA (NCP145)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.07
Spacegroup name	P 21 21 21
Unit cell lengths	105.606, 110.066, 181.719
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	91.000 - 2.500
R-factor	0.23701
Rwork	0.236
R-free	0.26972
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2nzd
RMSD bond length	0.009
RMSD bond angle	1.420
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	91.000
High resolution limit [Å]	2.500
Rmerge	0.059
Number of reflections	72054
<I/σ(I)>	21.6
Completeness [%]	99.6
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	289	KCl, MnCl2, K-Cacodylate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K