3REB
HIV-1 Nef protein in complex with engineered Hck-SH3 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-29 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9763 |
Spacegroup name | P 61 |
Unit cell lengths | 112.460, 112.460, 130.030 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.860 - 3.450 |
R-factor | 0.2171 |
Rwork | 0.216 |
R-free | 0.24260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rea |
RMSD bond length | 0.016 |
RMSD bond angle | 1.584 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC (5.5.0102) |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.550 |
High resolution limit [Å] | 3.450 | 3.450 |
Number of reflections | 12282 | |
<I/σ(I)> | 16.6 | |
Completeness [%] | 99.0 | 99 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 285 | 100 mM Hepes, 0.05 M sodium citrate, 15% isopropanol, 30% glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 285K |