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3REB

HIV-1 Nef protein in complex with engineered Hck-SH3 domain

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSLS BEAMLINE X10SA
Synchrotron siteSLS
BeamlineX10SA
Temperature [K]100
Detector technologyCCD
Collection date2009-05-29
DetectorMARMOSAIC 225 mm CCD
Wavelength(s)0.9763
Spacegroup nameP 61
Unit cell lengths112.460, 112.460, 130.030
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution19.860 - 3.450
R-factor0.2171
Rwork0.216
R-free0.24260
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)3rea
RMSD bond length0.016
RMSD bond angle1.584
Data reduction softwareXDS
Data scaling softwareXSCALE
Phasing softwareREFMAC (5.5.0102)
Refinement softwareREFMAC (5.5.0102)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]20.0003.550
High resolution limit [Å]3.4503.450
Number of reflections12282
<I/σ(I)>16.6
Completeness [%]99.099
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7285100 mM Hepes, 0.05 M sodium citrate, 15% isopropanol, 30% glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 285K

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