3RC0

Human SETD6 in complex with RelA Lys310 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-21
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97918
Spacegroup name	P 1
Unit cell lengths	61.621, 64.501, 73.759
Unit cell angles	78.73, 70.60, 64.21

Refinement procedure

Resolution	34.720 - 2.190
R-factor	0.1688
Rwork	0.166
R-free	0.23430
Structure solution method	MIR
Starting model (for MR)	3qxy
RMSD bond length	0.006
RMSD bond angle	0.950
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SGXPRO
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.720	2.270
High resolution limit [Å]	2.190	2.190
Rmerge	0.122	0.427
Number of reflections	48552
<I/σ(I)>	11.4	2.4
Completeness [%]	98.4	97.4
Redundancy	3.6	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.6	289	15% (w/v) polyethylene glycol (PEG) 3350, 0.1 M di-ammonium hydrogen citrate, pH 4.6, VAPOR DIFFUSION, temperature 289K