3RBQ
Co-crystal structure of human UNC119 (retina gene 4) and an N-terminal Transducin-alpha mimicking peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-04 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9749 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.546, 79.713, 189.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.370 - 2.000 |
| R-factor | 0.19134 |
| Rwork | 0.188 |
| R-free | 0.24557 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gqq |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.843 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 1.988 | 1.988 |
| Rmerge | 0.701 | |
| Number of reflections | 82342 | |
| <I/σ(I)> | 11 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 15.2 | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 5 | 277 | 40% PEG 5000, 0.1 M sodium acetate, 0.1 M potassium acetate, 30% isopropanol, pH 5.0, LIQUID DIFFUSION, temperature 277K |
