3RBG
Crystal structure analysis of Class-I MHC restricted T-cell associated molecule
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 116.020, 116.291, 79.018 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.470 - 2.300 |
| R-factor | 0.2001 |
| Rwork | 0.198 |
| R-free | 0.23350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z9m |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.442 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.360 |
| High resolution limit [Å] | 2.300 | 6.290 | 2.300 |
| Rmerge | 0.076 | 0.041 | 0.462 |
| Number of reflections | 23676 | ||
| <I/σ(I)> | 12.2 | ||
| Completeness [%] | 98.9 | 94.3 | 99.8 |
| Redundancy | 5.5 | 5.4 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 277 | 0.49 M Sodium Phosphate, 0.91M Potasium Phosphate pH 6.9, Vapor diffusion, Sitting drop, temperature 277K |
