3RBF
Crystal structure of Human aromatic L-amino acid decarboxylase (AADC) in the apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-22 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 175.840, 175.840, 74.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.770 - 2.900 |
| R-factor | 0.2147 |
| Rwork | 0.212 |
| R-free | 0.26680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1js6 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.263 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.770 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.112 | 0.479 |
| Number of reflections | 26454 | |
| <I/σ(I)> | 15.7 | 3.6 |
| Completeness [%] | 99.3 | 99.3 |
| Redundancy | 8.8 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 0.1 M HEPES pH 7.0, 28-30% Jeffamine ED 2001 pH 7.0, vapor diffusion, hanging drop, temperature 294K |
