3RAN
CANINE GDP-RAN Q69L MUTANT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-01-29 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 63.090, 59.170, 116.370 |
Unit cell angles | 90.00, 100.68, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.300 * |
Rwork | 0.176 |
R-free | 0.27100 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | EBI-1279 |
RMSD bond length | 0.012 * |
RMSD bond angle | 2.100 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.300 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.035 | 0.155 |
Total number of observations | 76123 * | |
Number of reflections | 35632 | |
<I/σ(I)> | 14.8 | 4.9 |
Completeness [%] | 94.7 | 94.9 |
Redundancy | 2.1 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.4 * | 18 * | SEE PUBLICATION, pH 7.2 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | PEG1000 | 35 (%) | |
3 | 1 | reservoir | 20 (mM) | ||
4 | 1 | reservoir | Tris-HCl | 50 (mM) |