3R90

Crystal structure of Malignant T cell-amplified sequence 1 protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-25
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97901
Spacegroup name	P 31
Unit cell lengths	115.590, 115.590, 157.790
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.420 - 1.700
R-factor	0.202
Rwork	0.202
R-free	0.23530
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.267
Data reduction software	DENZO
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	7.600	1.740
High resolution limit [Å]	1.700	5.380	1.700
Rmerge	0.084	0.041	0.869
Number of reflections	259245	5285	19130
<I/σ(I)>	13.33	34.6	1.9
Completeness [%]	99.9	99.5	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	2.5M ammonium sulfate, 0.1M Bis-Tris propane, pH 7, VAPOR DIFFUSION, temperature 293K
1	VAPOR DIFFUSION	7	293	2.5M ammonium sulfate, 0.1M Bis-Tris propane, pH 7, VAPOR DIFFUSION, temperature 293K