3R90
Crystal structure of Malignant T cell-amplified sequence 1 protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97901 |
| Spacegroup name | P 31 |
| Unit cell lengths | 115.590, 115.590, 157.790 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.420 - 1.700 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.23530 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.267 |
| Data reduction software | DENZO |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 7.600 | 1.740 |
| High resolution limit [Å] | 1.700 | 5.380 | 1.700 |
| Rmerge | 0.084 | 0.041 | 0.869 |
| Number of reflections | 259245 | 5285 | 19130 |
| <I/σ(I)> | 13.33 | 34.6 | 1.9 |
| Completeness [%] | 99.9 | 99.5 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 2.5M ammonium sulfate, 0.1M Bis-Tris propane, pH 7, VAPOR DIFFUSION, temperature 293K |
| 1 | VAPOR DIFFUSION | 7 | 293 | 2.5M ammonium sulfate, 0.1M Bis-Tris propane, pH 7, VAPOR DIFFUSION, temperature 293K |
