3R7T
Crystal Structure of Adenylosuccinate Synthetase from Campylobacter jejuni
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-18 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.97903 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 127.232, 127.232, 122.250 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.175 - 2.300 |
R-factor | 0.178 |
Rwork | 0.176 |
R-free | 0.20900 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.410 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 26582 | |
<I/σ(I)> | 13.2 | 5.75 |
Completeness [%] | 99.9 | 100 |
Redundancy | 23.5 | 23.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 0.2 M magnesium chloride 0.1 M sodium citrate pH 5.5, 40 % (v/v) PEG400, VAPOR DIFFUSION, SITTING DROP, temperature 289K |