3R7O

Structure of dually phosphorylated c-MET receptor kinase in complex with an MK-2461 analog

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2009-05-02
Detector	ADSC QUANTUM 210
Spacegroup name	P 21 21 21
Unit cell lengths	42.671, 64.875, 110.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.170 - 2.300
R-factor	0.2127
Rwork	0.211
R-free	0.23970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.220
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	BUSTER-TNT (BUSTER 2.9.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	200.000	200.000	2.350
High resolution limit [Å]	2.300	5.670	2.300
Rmerge	0.062	0.031	0.500
Number of reflections	14239
<I/σ(I)>	14.6
Completeness [%]	99.4	93.5	99.5
Redundancy	7	6.2	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	15.6 mg/ml protein mixed in 1:1 ratio with reservoir containing 150 mM malic acid, 20% PEG3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K