3R68
Molecular Analysis of the PDZ3 domain of PDZK1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-21 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 69.910, 69.910, 37.659 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.150 - 1.300 |
| R-factor | 0.13192 |
| Rwork | 0.131 |
| R-free | 0.15686 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.607 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.220 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Number of reflections | 28919 | |
| Completeness [%] | 97.0 | 69.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 291 | 0.2M Zinc acetate, 0.1M sodium cacodylate, pH 6.2, 15% (W/V) PEG 3350, 10% ethylene glycol, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
