3R5B
Pseudomonas aeruginosa DapD (PA3666) in complex with L-2-aminopimelate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.038, 102.002, 134.903 |
| Unit cell angles | 90.00, 89.97, 90.00 |
Refinement procedure
| Resolution | 36.880 - 2.510 |
| R-factor | 0.25149 |
| Rwork | 0.250 |
| R-free | 0.27567 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.242 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 37.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.144 |
| Number of reflections | 72498 |
| <I/σ(I)> | 6.7 |
| Completeness [%] | 95.9 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | 19-20% of PEG3350, 0.3-0.4M succinate, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
