3R5A
Pseudomonas aeruginosa DapD (PA3666) in complex with D-2-aminopimelate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-08 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.04 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.343, 101.931, 135.686 |
| Unit cell angles | 90.00, 89.97, 90.00 |
Refinement procedure
| Resolution | 21.830 - 1.890 |
| R-factor | 0.19755 |
| Rwork | 0.196 |
| R-free | 0.23532 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.145 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.000 | 1.980 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.059 | 0.333 |
| Number of reflections | 173938 | |
| <I/σ(I)> | 9.8 | 2.3 |
| Completeness [%] | 91.2 | 72.8 |
| Redundancy | 2.5 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | 19-20% of PEG3350, 0.3-0.4M succinate, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
