3R4L
Human very long half life Plasminogen Activator Inhibitor type-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-08-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 72.007, 72.007, 194.481 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.000 - 2.700 |
R-factor | 0.212 |
Rwork | 0.209 |
R-free | 0.28200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 9pai |
RMSD bond length | 0.015 |
RMSD bond angle | 1.860 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0093) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.003 | 2.800 |
High resolution limit [Å] | 2.699 | 2.699 |
Rmerge | 0.064 | 0.270 |
Number of reflections | 13852 | |
<I/σ(I)> | 18.1 | 2.5 |
Completeness [%] | 82.4 | 98.7 |
Redundancy | 4 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.025M NACL, 27% TERT-BUTANOL, 0.050M TRIS-HCL, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |