3R45
Structure of a CENP-A-Histone H4 Heterodimer in complex with chaperone HJURP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-01-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9788 |
Spacegroup name | P 3 1 2 |
Unit cell lengths | 83.941, 83.941, 86.773 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.386 - 2.600 |
R-factor | 0.1874 |
Rwork | 0.185 |
R-free | 0.24540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3nqj |
RMSD bond length | 0.006 |
RMSD bond angle | 0.838 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.6.2_432)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.094 | 0.416 |
Number of reflections | 10744 | |
<I/σ(I)> | 15.1 | 3.9 |
Completeness [%] | 98.0 | 99.4 |
Redundancy | 5.7 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289 | 2M ammonium sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |