3R3L
Structure of NP protein from Lassa AV strain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-02-07 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97745 |
Spacegroup name | P 3 |
Unit cell lengths | 176.320, 176.320, 56.580 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.449 |
R-factor | 0.18548 |
Rwork | 0.184 |
R-free | 0.21908 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mwp |
RMSD bond length | 0.006 |
RMSD bond angle | 0.960 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 88.160 |
High resolution limit [Å] | 2.440 |
Number of reflections | 37576 |
Completeness [%] | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | Protein at 7.5 mg/ml. Reservoir: 7% PEG300, 0.5 M KCl, 10% glycerol, VAPOR DIFFUSION, HANGING DROP |