3R3I
Crystal Structure of C-terminal truncation of UDP-glucose Pyrophosphorylase of Homo sapiens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-31 |
Detector | MAR |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 140.266, 140.266, 315.284 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.980 - 3.570 |
R-factor | 0.2353 |
Rwork | 0.233 |
R-free | 0.28620 |
Starting model (for MR) | 3r2w |
RMSD bond length | 0.013 |
RMSD bond angle | 1.559 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.981 | 3.610 |
High resolution limit [Å] | 3.566 | 3.566 |
Rmerge | 0.095 | 0.649 |
Number of reflections | 42839 | |
Completeness [%] | 97.9 | 61.2 |
Redundancy | 7.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | magnesium sulfate, PEG3350, glycerol, HEPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |