3R2W
Crystal Structure of UDP-glucose Pyrophosphorylase of Homo Sapiens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-15 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 140.445, 140.445, 311.724 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.600 |
| R-factor | 0.2502 |
| Rwork | 0.247 |
| R-free | 0.30380 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.558 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 120.000 | 3.690 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Number of reflections | 39283 | |
| Completeness [%] | 96.9 | 64.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | MgSO4, 35.8% PEG3350, glycerol, HEPES, pH 6.5, vapor diffusion, hanging drop, temperature 290K |
