3R25

Crystal structure of enolase superfamily member from Vibrionales bacterium complexed with Mg and Glycerol in the active site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-18
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97915
Spacegroup name	I 2 2 2
Unit cell lengths	167.986, 211.516, 211.672
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.036 - 1.603
R-factor	0.1623
Rwork	0.161
R-free	0.18590
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.980
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	BALBES
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall
Low resolution limit [Å]	39.036
High resolution limit [Å]	1.603
Number of reflections	477578
Completeness [%]	98.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.5 M magnesium formate, 0.1 M bis-tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K