3QZ2

The structure of cysteine-free human insulin degrading enzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	278
Detector technology	IMAGE PLATE
Collection date	2010-10-19
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.9597
Spacegroup name	P 65
Unit cell lengths	262.073, 262.073, 90.838
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 3.200
R-factor	0.17514
Rwork	0.172
R-free	0.24072
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	1.852
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASES
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.200
Number of reflections	55620
Completeness [%]	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	10-13% PEG MME 5000, 100 mM HEPES pH 7.0, 4-14% Tacsimate, 10% dioxane, VAPOR DIFFUSION, HANGING DROP, temperature 298K