3QY9
The Crystal Structure of Dihydrodipicolinate reductase from Staphylococcus aureus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-01 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97625 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 85.900, 112.440, 131.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.500 - 1.800 |
R-factor | 0.199 |
Rwork | 0.198 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1arz |
RMSD bond length | 0.007 |
RMSD bond angle | 1.046 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.640 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.086 | 0.559 |
Number of reflections | 115798 | |
<I/σ(I)> | 12.7 | 2.9 |
Completeness [%] | 98.0 | 93.4 |
Redundancy | 6.9 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microbatch under oil sitting drop | 4.5 | 298 | 2.0M Ammonium sulfate, 0.2M Sodium actetate , pH 4.5, Microbatch under oil sitting drop, temperature 298K |