3QY9
The Crystal Structure of Dihydrodipicolinate reductase from Staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.900, 112.440, 131.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.500 - 1.800 |
| R-factor | 0.199 |
| Rwork | 0.198 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1arz |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.046 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.640 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.086 | 0.559 |
| Number of reflections | 115798 | |
| <I/σ(I)> | 12.7 | 2.9 |
| Completeness [%] | 98.0 | 93.4 |
| Redundancy | 6.9 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch under oil sitting drop | 4.5 | 298 | 2.0M Ammonium sulfate, 0.2M Sodium actetate , pH 4.5, Microbatch under oil sitting drop, temperature 298K |
