3QXU
Free Structure of an Anti-Methotrexate CDR1-3 Graft VHH Antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-17 |
| Detector | MAR 225 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 61 |
| Unit cell lengths | 101.087, 101.087, 156.852 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.946 - 1.800 |
| R-factor | 0.18345 |
| Rwork | 0.182 |
| R-free | 0.21411 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.030 |
| RMSD bond angle | 1.427 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 19.946 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.132 |
| Number of reflections | 91151 |
| <I/σ(I)> | 13 |
| Completeness [%] | 99.8 |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | 3.5M Sodium Formate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
