3QT2

Structure of a cytokine ligand-receptor complex

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-28
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9079, 0.9795, 0.9797
Spacegroup name	P 1 21 1
Unit cell lengths	85.084, 61.633, 142.113
Unit cell angles	90.00, 99.49, 90.00

Refinement procedure

Resolution	42.020 - 2.550
R-factor	0.21025
Rwork	0.207
R-free	0.26442
Structure solution method	MAD
RMSD bond length	0.019
RMSD bond angle	1.628
Data reduction software	CrystalClear
Data scaling software	CrystalClear
Phasing software	SHARP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	140.030	2.640
High resolution limit [Å]	2.550	2.550
Rmerge		0.445
Number of reflections	91854
<I/σ(I)>	8.2	1.9
Completeness [%]	99.9	100
Redundancy	2.53	2.53

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	10% (w/v) PEG 20000, 0.1M MOPS pH 6.5, 20% (w/v) glucose, 2.5% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 294K