3QPW
PFKFB3 in complex with Aluminum Tetrafluoride
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CAMD BEAMLINE GCPCC |
Synchrotron site | CAMD |
Beamline | GCPCC |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-26 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.3808 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 102.709, 102.709, 257.044 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.250 |
R-factor | 0.20162 |
Rwork | 0.200 |
R-free | 0.24216 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.022 |
RMSD bond angle | 1.814 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | REFMAC (5.2.0019) |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.330 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 38530 | |
Completeness [%] | 99.5 | 98.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 300 | 00 mM Tris-HCl, pH 7.5, 20-25% ethylene glycol, 200-400mM Tartaric Acid, 5% glycerol,12% polyethylene glycol 4000, VAPOR DIFFUSION, SITTING DROP, temperature 300K |